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American Society of Microbiology ABMM Sample Questions:

1. A patient develops a severe diarrheal illness after consuming raw seafood. Stool culture yields a Gram- negative, comma-shaped bacterium that is oxidase-positive and grows on thiosulfate-citrate-bile salts-sucrose (TCBS) agar, producing blue-green colonies. The MOST significant virulence factor contributing to the severe, cholera-like diarrhea caused by this organism is:

A) Cholera toxin (CT)-like enterotoxin
B) Type III secretion system (T3SS)
C) Heat-labile toxin (LT)
D) Heat-stable enterotoxin (ST)

2. Metagenomic analysis of a bronchoalveolar lavage (BAL) sample from a patient with severe pneumonia reveals a complex microbial community with a high abundance of both bacterial and fungal sequences. This approach is particularly useful in identifying:

A) The presence of fastidious or unculturable pathogens that may be missed by traditional methods.
B) The specific antibiotic susceptibility profile of the predominant bacterial pathogen.
C) The presence of specific virulence factors expressed by the identified pathogens.
D) The relative proportions of different immune cell populations in the BAL fluid.

3. Chagas disease, characterized by acute Romana's sign and potential chronic cardiac and gastrointestinal complications, is caused by which vector-borne protozoan parasite?

A) Leishmania donovani
B) Trypanosoma cruzi
C) Toxoplasma gondii
D) Trypanosoma brucei gambiense

4. An immunocompromised patient develops a disseminated fungal infection. Blood cultures are negative, but a serum galactomannan assay is strongly positive. A CT scan of the chest reveals multiple nodular lesions with a halo sign. Bronchoalveolar lavage (BAL) fluid is analyzed by PCR, yielding a positive result for Aspergillus fumigatus. The halo sign observed on the CT scan is primarily attributed to:

A) A hypersensitivity reaction in the lung parenchyma.
B) Formation of thick-walled cysts containing fungal conidia.
C) Angioinvasion by Aspergillus hyphae leading to localized hemorrhage and necrosis.
D) Extensive granuloma formation surrounding fungal hyphae.

5. A patient with a history of travel to South America presents with fever, lymphadenopathy, and hepatosplenomegaly. Peripheral blood smear reveals the presence of trypomastigotes. The MOST likely diagnosis is:

A) Leishmaniasis
B) Chagas disease (American trypanosomiasis)
C) Malaria
D) African trypanosomiasis (sleeping sickness)

Solutions: