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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is investigating a cluster of bloodstream infections caused by Staphylococcus aureus. To differentiate between community-associated (CA-MRSA) and healthcare- associated (HA-MRSA) strains, they may look for the presence of:

A) Specific staphylococcal cassette chromosome mec (SCCmec) types, such as SCCmec IV or V inCA- MRSA.
B) Resistance to non-beta-lactam antibiotics, which is more common in CA-MRSA.
C) Panton-Valentine leukocidin (PVL) toxin genes, which are exclusively found in HA-MRSA.
D) The mecA gene, which is common to both CA-MRSA and HA-MRSA.

2. An acid-fast bacillus isolate recovered from a respiratory specimen after 3 weeks of incubation on Löwenstein- Jensen medium is initially non-pigmented when grown in the dark. After exposure to light for several hours, the colonies develop a distinct yellow-orange pigment. This organism is classified as a:

A) Nonchromogen
B) Scotochromogen
C) Photochromogen
D) Rapid grower

3. Lyme disease, characterized initially by erythema migrans, is caused by Borrelia burgdorferi. What is the primary arthropod vector responsible for transmitting this spirochete to humans in the Northeastern United States?

A) Pulex irritans (Human flea)
B) Dermacentor variabilis (American dog tick)
C) Amblyomma americanum (Lone Star tick)
D) Ixodes scapularis (Blacklegged tick)

4. A clinical microbiology laboratory is implementing a new real-time PCR assay for the direct detection of Chlamydia trachomatis in urine samples. To ensure the accuracy of negative results, it is essential to include:

A) A high concentration of the Chlamydia trachomatis target sequence.
B) An internal amplification control to detect PCR inhibition.
C) A positive control containing Neisseria gonorrhoeae DNA.
D) A melting curve analysis to confirm the specificity of the amplicon.

5. The erm gene in Staphylococcus aureus confers resistance to which class(es) of antibiotics via modification of the ribosomal target site?

A) Aminoglycosides
B) Macrolides, Lincosamides, and Streptogramin B (MLS<sub>B</sub>)
C) Fluoroquinolones
D) Beta-lactams only

Solutions: